Table 1.
Human primer sequences specially designed for real-time RT-polymerase chain reaction (PCR) amplification.
| Gene | Forward/Reverse | Primer sequence | Accession no. | Size of product (bp) | Melting temperature (°C) |
|---|---|---|---|---|---|
| c-Fos | F | 5' AGA ATC CGA AGG GAA AGG AA 3' | NM_005252 | 150 | 63.6 |
| R | 5' CTT CTC CTT CAG CAG GTT GG 3' | 63.8 | |||
| c-Jun | F | 5' CAG GTG GCA CAG CTT AAA CA 3' | NM_002228 | 80 | 63.8 |
| R | 5' GTT TGC AAC TGC TGC GTT AG 3' | 63.5 | |||
| MMP-13 | F | 5' CTT CCC AAC CGT ATT GAT GC 3' | NM_002427 | 143 | 64.1 |
| R | 5' TTT GGA AGA CCC AGT TCA GA 3' | 62.2 | |||
| Runx2 | F | 5' TCT GGC CTT CCA CTC TCA GT 3' | NM_004348 | 142 | 64.0 |
| R | 5' AAG GTG GCT GGA TAG TGC AT 3' | 63.4 | |||
| RPS18 | F | 5' GAT GGG CGG CGG AAA ATA G 3' | NM_022551 | 165 | 68.4 |
| R | 5' GCG TGG ATT CTG CAT AAT GGT 3' | 65.8 | |||
| GAPDH | F | 5' CAT GAG AAG TAT GAC AAC AGC CT 3' | NM_002046 | 113 | 62.0 |
| R | 5' AGT CCT TCC ACG ATA CCA AAG T 3' | 62.5 |