Figure 1.

(a) Age-dependent expression of FasL in the rat testis. Total RNA (20 μg/lane) extracted from total testis from animals of various ages were analyzed by Northern blot. Nylon filter was hybridized with ³²P-labeled rat FasL cDNA probe (b) Northern blot analysis of FasL expression in Sertoli cells at different ages. Total RNA (20 μg/lane) from cultured and crude (*) Sertoli cells were subjected to Northern blot analysis for detection of rat FasL. N2a-FasL+ and N2a-Neo cell lines were used as positive and negative controls for FasL, respectively. The integrity and equal loading of RNA was ascertained by ethidium bromide staining of the gel before transfer (Lower). Data are representative of three independent experiments.