Figure 4.

Evaluation of DNA content of different testicular cell types by propidium iodide incorporation. (a–c) The percentage of gated cells with indicated ploidy is shown in brackets. Flow cytometric analysis of FasL expression in the seminiferous epithelium and in activated T lymphocytes. The expression of FasL was determined by biotin-conjugated anti-FasL monoclonal antibody (MFL4). (d–o) The white histograms indicate isotype control and gray histograms indicate the staining with specific antibody. l and m show negative and positive controls for FasL, respectively. Results are representative of three independent experiments.