(A–B) Typical full MS spectra of newly synthesized peptides and original peptides after (A) 4 hr and (B) 48 hr transfection of microRNA. The triplet peptide sequence, m/z, and charge status were indicated accordingly. The SILAC-L, SILAC-M, and SILAC-H peptides indicate the original peptides (light), newly synthesized peptides after microRNA transfection (medium-heavy), and newly synthesized peptides after GFP transfection (heavy). (C–D) Correlation of peptides H/M log-ratios (heavy/medium-heavy, log2 based) quantified by UNiquant for the 2 replicates in the (C) 4 hr and (D) 48 hr triple pSILAC dataset.