Table 2.
Amplicons [CpG site(s) included] | Initial denaturing T (°C), T (min.) | Cycles (X45) |
Final elongation T (°C), T (min.) | ||
---|---|---|---|---|---|
denaturing T (°C), T (s) | annealing T (°C), T (s) | elongation T (°C), T (s) | |||
−295 | 94,15 | 94,30 | 58,30 | 72,30 | 72,10 |
−186 | 94,15 | 94,30 | 54,30 | 72,30 | 72,10 |
−54 | 94,15 | 94,30 | 60,30 | 72,30 | 72,10 |
+122–+128 | 94,15 | 94,30 | 55,30 | 72,30 | 72,10 |
+171 | 94,15 | 94,30 | 55,30 | 72,30 | 72,10 |