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Bisulphite-specific polymerase chain reaction conditions for CpG containing amplicons
| Amplicons [CpG site(s) included] | Initial denaturing T (°C), T (min.) | Cycles (X45) |
Final elongation T (°C), T (min.) | ||
|---|---|---|---|---|---|
| denaturing T (°C), T (s) | annealing T (°C), T (s) | elongation T (°C), T (s) | |||
| −295 | 94,15 | 94,30 | 58,30 | 72,30 | 72,10 |
| −186 | 94,15 | 94,30 | 54,30 | 72,30 | 72,10 |
| −54 | 94,15 | 94,30 | 60,30 | 72,30 | 72,10 |
| +122–+128 | 94,15 | 94,30 | 55,30 | 72,30 | 72,10 |
| +171 | 94,15 | 94,30 | 55,30 | 72,30 | 72,10 |
