Figure 7.

Age-related changes in nuclear SIRT1 in wake–active neurons. A, LC neurons across ages 2 (2M), 12 (12M), and 24 (24M) months. Left, Anti-tyrosine hydroxylase (red) and anti-SIRT1 (green) labeling for the three age groups in LC sections. Right, Autofluorescence (yellow) in non-immunolabeled sections in LC, counterstained with 4′,6-diamidino-2-phenylindole (DAPI). B, Mean ± SE age effect on nuclear SIRT1 immunodensity in LC neurons. Lines signify statistical significances (p < 0.01). C, Age-related SIRT1 immunoreactivity in dopaminergic ventral periaqueductal gray wake neurons for 2 and 12 months. Arrow highlights dendritic SIRT1 at 12 months.