TABLE 2.
Effect of BLM expression on GCR accumulation in the sgs1Δ mutant.
| Relevant Genotype1 | Galactose concentration in media | GCR Rate (Canr 5-FOAr × 10−10) | 95% CI2 (Canr 5-FOAr × 10−10) |
|---|---|---|---|
| wildtype | 0% | 1.1 | < 1 – 6.2 |
| PSGS1BLM | 0% | 70 | 56–151 |
| PGALBLM | 0% | 61 | 30–153 |
| PGALBLM | 0.1% | 335 | 233–576 |
| PGALBLM | 0.5% | 382 | 170–777 |
| PGALBLM | 2% | 1832 | 1090–2910 |
| PGALSGS1 | 2% | < 11 | < 9–12 |
| sgs1Δ | 2% | 54 | 23–104 |
Human BLM cDNA was inserted at the endogenous SGS1 locus, fused to the native SGS1 promoter (PSGS1) or fused to a galactose-inducible promoter (PGAL). In PGALSGS1, the native SGS1 promoter region was disrupted by fusing the SGS1 ORF to a galactose-inducible promoter. If strains expressing BLM or SGS1 genes from the galactose-inducible GAL1 promoter were grown in less than 2% galactose (to lower protein expression levels) media was supplemented with sucrose to reach a total sugar concentration of 2%.
95% confidence intervals (CI) were calculated according to Nair 59.