Effect of adding NO on 3-NT and nitrite formation in albumin solution in hyperoxia. a 3-Nitrotyrosine formation in a 10% albumin solution bubbled with either O2 or O2 + NO. The 3-NT densities for each time point are corrected for the protein concentrations at each time point, and the fraction of normalized 3-NT levels are expressed as the fraction of 3-NT content at time 0. n = 4 for O2 and n = 3 for O2 + NO. * O2 alone different from O2 + NO, p < 0.05. b The amount of nitrite formed per milligram of protein in the 10% albumin solution after 0, 1, and 2 h of exposure to either O2 alone or O2 + NO. * O2 + NO different from O2 alone, p < 0.05