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. Author manuscript; available in PMC: 2011 Nov 9.
Published in final edited form as: Mol Cancer Ther. 2010 Nov 9;9(11):2970–2981. doi: 10.1158/1535-7163.MCT-10-0780

Figure 4. Expression of Tau exons 4A, 6 and 8.

Figure 4

Total RNA was extracted from each cell line, reverse transcribed to cDNA and amplified by polymerase chain reaction to test for the presence of (A) exon 6 and 8 using primers spanning exons 5 and 9; (B) exon 6 in Tau 0N isoforms; (C) exon 6 in Tau 1N isoforms; (D) exon 6 in Tau 2N isoforms. Each PCR reaction co-amplified housekeeping gene TFRC as an internal and loading control. The arrows point to all possible products given the particular primer pair.