Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Pediatr Res. 2011 Mar;69(3):212–216. doi: 10.1203/PDR.0b013e3182084f8d

Table 2.

Real Time polymerase chain reaction (PCR) measurements of RNA expressed by Right Middle Lobe from LIGATION and CONTROL preterm newborn animals

Gene Newborn-14 d No Ligation Newborn-14 d Ligation Comparison
ΔCT (MDH – gene) ΔCT (MDH – gene) Newborn-Ligation versus Newborn-No Ligation
mean SD mean SD p<0.05
Inflammation
CD14 −2.358 0.427 −1.776 0.176 graphic file with name nihms279446ig1.jpg
COX-2 −5.429 0.551 −4.561 0.474 graphic file with name nihms279446ig1.jpg
IL-6 −6.981 1.002 −6.376 0.599 -
IL-8 −4.352 0.698 −4.298 0.262 -
TNFalpha −7.983 0.795 −7.457 0.593 graphic file with name nihms279446ig1.jpg
Epithelial water reabsorption
Alpha-ENaC −2.161 0.533 −2.533 0.079 graphic file with name nihms279446ig2.jpg
Vascular growth and contractility
ANGPT2/angiopoietin-2 −6.987 0.497 −7.465 0.362 graphic file with name nihms279446ig2.jpg
eNOS −6.183 0.450 −6.173 0.261 -
ETA receptor −0.756 0.385 −0.876 0.156 -
CDH5/VE-cadherin −0.748 0.367 −0.933 0.154 -
VEGF −0.370 0.376 −0.607 0.295 -
VEGFR1/FLT-1 −2.810 0.415 −3.113 0.253 -
VEGFR2/KDR −0.917 0.428 −0.929 0.250 -
Remodeling
HAS-2 −7.857 1.703 −7.109 1.430 graphic file with name nihms279446ig1.jpg
HIF2 alpha 1.920 0.412 2.092 0.196 -
MMP-9 −4.461 0.579 −4.105 0.336 -
RHAMM −5.147 0.558 −5.289 0.391 -
TGFbeta1 −2.760 0.450 −2.996 0.072 -
TGFbeta3 −2.707 0.324 −3.125 0.374 graphic file with name nihms279446ig2.jpg
Surfactant
SP-B 5.865 0.372 5.895 0.210 -

See legend to Table 1 for explanation of ΔCT. The more negative the ΔCT, the fewer the number of starting copies of a gene (mRNA). Number of separate animals used: CONTROL (Newborn-14 d No Ligation ) (n=12); LIGATION (Newborn-14 d Ligation) (n=7). Newborn-Ligation versus Newborn-No Ligation: Right Middle lobes from 14 days old newborns (whose PDA were ligated) were compared with Right Middle lobes from newborns (whose PDA were NOT ligated). Inline graphic p<0.05, LIGATION Right Middle Lobe ΔCT was significantly greater than CONTROL Right Middle Lobe ΔCT; Inline graphic p<0.05, ΔCT was significantly less; - p>0.05, was not significantly different.