Table 2.
Batch balance for Thermotoga maritima grown on BM under anoxic conditions including the oxidative period.
| Phase | Oxygen addition | Glucose consumed (mM) | Biomass (mg cdw L−1) | Fructose produced (mM) | End products of metabolism (mM) | Carbon balance (%) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Acetate | Lactate | Alanine | EPS | CO2 | H2 | ||||||
| Growth | − | 5.46 | 81.4 ± 17 | 1.10 | 5.60 | 0.91 | 0.7 ± 0.2 | 1.22 ± 0.7 | 6.53 | 9.91 | 111.4 ± 19 |
| Oxidative stationary growth | + | 4.86 | − | 0.71 | 2.52 | 1.96 | 0.1 ± 0.2 | nd | 5.13 | 3.22 | 70.7 ± 18 |
| Stationary growth | − | 8.30 | − | 0.00 | 10.15 | 2.58 | 0.5 ± 0.2 | nd | 8.63 | 18.62 | 76.3 ± 13 |
| Overall fermentation | 19.3 | 81.4 ± 17 | 1.81 | 18.27 | 5.45 | 1.3 ± 0.2 | 1.22 ± 0.7 | 20.29 | 31.75 | 88.7 ± 11 | |
Fermentation runs were conducted at 80°C under anoxic conditions using the BM.
Growth phase (0–14 h); oxidative stationary growth phase (14–44.6 h); anoxic stationary growth phase (44.6–114.2).
cdw: cell dry weight.
EPS: Extracellular polysaccharides.