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. 2011 Apr;22(4):664–672. doi: 10.1681/ASN.2010060594

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Copyright © 2011 by the American Society of Nephrology

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Figure 6. — COX2 inhibition suppresses the enhanced expression of PCNA in PTGs of uremic rats. (A) Immunohistochemistry with antibody for COX2 and PCNA in PTGs, showing enhanced staining of COX2 and PCNA in the chief cells of PTGs from uremic rats. Magnifications: ×400. (B) Immunoblot of PTGs using antibodies for PCNA/COX2/COX1 and β-actin as a loading control. (C) Real-time PCR analysis for PCNA/COX2/COX1 mRNA of RNA extracted from microdissected PTGs. The expression of COX2 and PCNA in PTGs was markedly increased in uremic rats fed with a high-phosphate diet compared with the sham group. COX2 inhibitor celecoxib significantly reduced PCNA protein and mRNA expression. Values are mean ± SEM. ^cP < 0.001 versus sham group; ^dP < 0.05 versus Nx + vehicle group; ^eP < 0.001 versus Nx + vehicle group.