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. 2011 Apr;22(4):673–680. doi: 10.1681/ASN.2010050468

Figure 3.

Figure 3.

IN-IC cells express V1aR and acid-base-related transporters but lack V2R (A) Photograph of IN-IC cells. Homogeneous cells with large nucleus are observed. (B) Expression of vasopressin and aldosterone-related receptors, channels and transporters in the IN-IC cells (top panel), and rat renal medulla (bottom panel). Although all of the examined receptors, transporters,and channels are present in rat renal medulla, V2R, aquaporin 2,and ENaC β and γ were not expressed in the IN-IC cells. Lane 1, size marker; lane 2, GAPDH (308 bp); lane 3, V1aR (425 bp); lane 4, V2R (578 bp); lane 5, aquaporin 2 (553 bp); lane 6, ENaC α (647 bp); lane 7, ENaC β (619 bp); lane 8, ENaCγ (561 bp); lane 9, size marker; lane 10, H-ATPase α1 (Hα1, 510 bp); lane 11, H-ATPase β1 (Hβ1, 503 bp); lane 12, H-ATPase β2 (Hβ2, 540 bp); lane 13, H-K-ATPase α1 (HKα1, 438 bp); lane 14, H-K-ATPase α2 (HKα2, 472 bp); lane 15, Rhcg (307 bp); lane 16, AE1 (178 bp); lane 17, pendrin (488 bp); lane 18, Foxi 1 (516 bp); lane 19, mineralocorticoid receptor (MR, 380 bp); lane 20, 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2, 361 bp). (C) Vasopressin decreased V1aR mRNA expression in IN-IC cells. Expression of mRNA without vasopressin was considered as 1. (D) Aldosterone dose-dependently decreased V1aR mRNA expression in IN-IC cells. Expression of mRNA without aldosterone was considered as 1. *P < 0.05 versus without vasopressin (C) or aldosterone (D). Means ± SEM. n = 4 to 6.