Figure 4.

Stimulative effects of arginine vasopressin and inhibitory effects of V1aR gene knockdown on the expression of acid-base–related transporters in IN-IC cells. Vasopressin increased the expression of H-ATPase, H-K-ATPase, and Rhcg in a dose-dependent manner. The effects of vasopression were almost completely abolished by a V1aR RNA interference-mediated knockdown experiment. Vasopressin did not affect the expression level of AE1 and pendrin. (A) H-ATPase α (116 kD), (B) H-K-ATPase β (67 kD), (C) Rhcg (58 kD), (D) AE1 (96 kD), (E) pendrin (100 kD), and (F) β actin (42 kD). Expression of each transporter without vasopressin in the absence or presence of siRNA was considered as 1. *P < 0.05 versus the expression in the absence of siRNA. Mean ± SEM. n = 5 to 9.