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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: FEBS Lett. 2011 Jan 23;585(4):671–676. doi: 10.1016/j.febslet.2011.01.030

Fig. 4.

Fig. 4

Retention of intron 1 sequences in TF mRNA and the involvement of Σ RNA/MALAT-1. HeLa cells were transfected with 75nM ASO254 against Σ RNA/MALAT-1 or control ASO. Twenty-four hrs after transfection, total RNAs were harvested. (A) Northern analyses. UTC, untreated control. Top panels show knockdown of Σ RNA/MALAT-1 by ASO254. Middle panels show three distinguished bands of TF transcripts, a major ~2.2-kb (TF) and two minor ~3-kb forms (iTF). Bottom panels show GAPDH as loading controls. Molecular size markers are shown to the right. (B) Quantitative results of iTF (both forms) based on Northern analyses normalized with GAPDH levels, mean ± SD, from 3 independent experiments.