Involvement of Σ RNA/MALAT-1 in TF alternative splicing in LPS-treated HUVEC. HUVEC were transfected with 75nM ASO254 against Σ RNA/MALAT-1 or control ASO for 24 hrs, and treated with 10 μg/ml LPS for 4 hrs. (A) Northern analyses (middle for Σ RNA/MALAT-1, and bottom for GAPDH), or RT-PCR for exons 4-6 of F3 (top). Molecular size markers are shown to the right. (B) Quantitative results of the ratio of hTFalt/GAPDH transcripts, mean ± SD, based on the RT-PCR results. (C) Ratio of hTFalt/GAPDH transcripts based on qPCR results, mean ± SD.