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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Arthritis Rheum. 2010 Sep;62(9):2707–2715. doi: 10.1002/art.27558

Figure 3.

Figure 3

Effect of oxygen tension and HIF on ANK promoter activity in nucleus pulposus (NP) cells. A. NP cells were transfected with ANK reporter constructs and luciferase reporter activity was measured in normoxia (21% O2) or hypoxia (1% O2). NP cells did not evidence hypoxic change in ANK promoter activity. Cells were transfected with B) HIF-1α-SiRNA (SiHIF-1α) C) HIF-2α-SiRNA (SiHIF-1α) and cultured in normoxia (Nx) and hypoxia (Hx). Control cells were transfected with control siRNA (C). Silencing of HIF-1α or HIF-2α resulted in a significant induction of ANK promoter activity. Induction was more pronounced in hypoxia. D) Effect of hypoxia on ANK promoter activity in HIF-1β wild type (WT) and null (KO) MEFs. In contrast to wild type cells, hypoxia induced ANK promoter activity in null cells. Values shown are mean ± SE from three independent experiments, * p < 0.05.