Table 3.

Epitopes identified by IgG reactivity detected in the spot-synthesis assay with serum from positive responders against the PvMSP-3α recombinant proteins.

PvMSP-3α Region	Epitopes	AA position	Position in Spot- synthesis assay
PvMSP3-Nt	EAPNSSRHHLRNGF	E₃₂-F₄₆	A8-A10
	KNDSLPHEEPNNLE	K₄₉-E₆₂	A12-A13
	DQVTKKEKKTIKKA	D₇₇-A₉₀	A19-A21
PvMSP3-BLI	AEQIQAELQKVKTA	A₁₀₅-A₁₁₈	B1-B4
	SATAAETAKNNAVS	S₁₂₅-S₁₃₈	B8
	KGLDAAKTAIKKAKAAAEEAKKEAAI	K₁₄₁-I₁₆₆	B11-B16
	KAEKDAEAAQ	K₁₆₈-Q₁₇₈	B19
	AKTAATNAEKKKTK	A₁₉₃-K₂₀₆	C1-C2
	EAQKACEKAKKAHA	K₂₂₉-A₂₄₂	C10-C11
	KSTEDLSVAKDK	K₂₈₂-K₂₉₄	C23-C24
	EIAAEVAKAKVAK	E₃₁₃-K₃₂₆	D2-D4
	KKAEEAKKIVDKI	K₃₃₃-I₃₄₅	D6-D7
	AAEFATEVKKAT	A₃₅₇-T₃₇₀	D11-D13
	GAKKAAGEAKKASI	G₄₀₁-I₄₀₉	E5
PvMSP3-BLII	EVAKAEVLNAEVK	E₄₂₁-K₄₃₄	E10-E11
	NDATEAKKQAEKAKAAAEEAKTHGEK	N₄₄₁-K₄₆₆	E14-E18
	KAYAVEAHLAKTKN	K₅₀₅-N₅₁₈	F2-F5
	DAANIAHQKWLKAT	D₅₄₁-T₅₅₄	F14-F19
	KAQKEATAAKLKA	K₅₇₃-A₅₈₅	F23-F24
	AEDAAEEAKEAAKK	A₆₄₅-K₆₅₈	G7-G12
	DKTIAAAKKAKKARE	D₆₇₃-E₆₈₆	G24-H2
PvMSP3-Ct	KAAYGLLKTKNQYVL	K₆₈₇-L₇₀₂	H3-H7
	LDISPESADNITSK	L₇₀₅-K₇₁₈	H8-H9
	QTGGNRERRNTSDTVDDT	Q₇₇₃-T₇₉₀	I1-I4
	GDEFDTYDDIKKVT	G₇₉₆-T₈₁₀	I6-I8