Figure 3.

Effects of FXR siRNA on pancreatic cancer cell proliferation, migration, and invasion. BF, basal FXR; CS, control siRNA; FS, FXR siRNA; MMC, mitomycin C. (A) MIA-PaCa2 and PANC-1 cells were transfected with control siRNA or FXR siRNA for the indicated time periods before cell number were estimated using CCK-8 assay. (B, C) MIA-PaCa2 and PANC-1 cells transfected with control siRNA or FXR siRNA were cultured in the presence of MMC (μg ml⁻¹) for 48 h. Cell were placed in serum-free culture media and added into the upper compartment of a migration or invasion chamber. After 24 h, cells in the upper chamber were removed and cells that had migrated (B) or invaded (C) onto the lower surface of the membrane were fixed and stained with Wright–Giemsa. The relative-fold migration and invasion values of FXR siRNA-transfected cells was normalised against control siRNA-transfected cells and expressed as percentages of the control, which was assumed to be 100%. Columns, mean of three independent experiments; bars, s.e.m. ^**P<0.01.