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. 2011 Mar 1;104(6):1027–1037. doi: 10.1038/bjc.2011.37

Figure 6.

Figure 6

Effect of FXR agonists GW4064 on pancreatic cancer cell migration and invasion. DMSO, control. (A, B) MIA-PaCa2 and PANC-1 cells were treated with 1 μM GW4064 for 48 h. Cells were placed in serum-free culture media and added into the upper compartment of a migration or invasion chamber. After 24 h, cells in the upper chamber were removed and cells that had migrated (A) or invaded (B) onto the lower surface of the membrane were fixed and stained with Wright–Giemsa. The relative-fold migration and invasion values of GW4064-treated cells were normalised against DMSO-treated cells and expressed as percentages of the control, which was assumed to be 100%. Columns, mean of three independent experiments; bars, s.e.m. *P<0.05.

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