Fig. 3.

The effect of immunization routes on the magnitude of induced CD8 T cell responses. C57BL/6 mice (three mice in each group) were immunized with either 1 million TU of OVA-lvv or HBS-lvv through intramuscular (im), footpad (fp), intravenous (iv), or intraperitoneal (ip). Eight days later, mice were sacrificed and splenocytes were collected and restimulated in vitro for 3 h with either SIINFEKL or HBV S Ag S190–198 peptide. Cells were then intracellularly stained for IFN-γ.