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. 2011 Mar 23;31(12):4755–4764. doi: 10.1523/JNEUROSCI.6594-10.2011

Figure 3.

Figure 3.

U0126 increases calcium level in axons during electrical stimulation. Ai, Left and middle, Images of axons of a TNXXL-transfected neuron with representative ROIs (colored squares) acquired using the CFP (left) and FRET (middle) channels (defined in Materials and Methods). Right, Dendrites with spines (indicated by arrows). Aii, Left and middle, corresponding dF/F0 for the color-matched ROIs in Ai; the black line represents average response. Right, Averaged dR/R0 (%) for all ROIs. Bi, Experimental scheme for testing effect of U0126 on FRET ratio. Pink vertical bars show times of imaging. Bii, Biii, [FRET ratio]4–6 for individual boutons (Bii) and averages for individual cells (Biii); lines connect data points for the same boutons/cell. Biv, dR/R0 for cells before (0–6 s), during (6–12 s), and after (12–23 s) electrical stimulation. Each data point is separated by 1 s. Horizontal bar on top of the graphs show duration of electrical stimulation. Number of cells/boutons is shown in parentheses. Data are presented as means ± SEM; **p < 0.01, ***p < 0.001 (black asterisks), for comparison between U0126 and DMSO. *p < 0.05, ***p < 0.001 (red asterisks), for comparison between washout and DMSO.