Figure 4.
Acute block of MET channel by 2 mm DHS. A, Representative trace of average microphonics before (middle; control) or 60 s after application of 2 mm DHS (bottom) in response to 20 Hz stimulus (top). B, Power spectra for control (black; n = 4), 2 mm DHS-treated (red; n = 4), and cdh23tc317e mutant (brown; n = 3) larvae. Note the characteristic 2f frequency component representing the neuromast's two direction-selective populations of hair cells. Error represented by shaded area above and below colored line. Power of averaged noise traces is indicated by dashed black line. C, A single recording with each trace showing an overlay of 10 consecutive sweeps (vertical calibration: 30 pA). Top, Twenty hertz stimulus trace; middle, control spikes (198 spikes); bottom, following 2 mm DHS application (35 spikes). D, Period histogram for all spikes from control (black bars) and following application of 2 mm DHS (red bars) from sixty 1 s sweeps of 20 Hz stimulation (from the cell in C). E, Average vector strength (gray circles; red and black circles are from the cell in C and D) for spikes during control and following 2 mm DHS application. The mean 20 Hz vector strength (error bar, SEM) for spontaneous activity is depicted by the white bar (data from Fig. 1).