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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 26;400(4):511–516. doi: 10.1016/j.bbrc.2010.08.081

Table 2. Activity and turnover number (kcat) of GS enzymesa.

Enzyme kcat (sec-1) % Activity
Wild-type 18.83 ± 0.77 100
G369V 0.133 ± 0.001 0.69
G370V 0.052 ± 0.026 0.27
G371V 2.36 ± 0.35 13.1
a

Results are an average of 3 different enzyme preparation, each assayed in duplicate. Control rates (lacking GAB) were subtracted to obtain kcat values. The lower limit of detection is 0.008 sec-1.