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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 26;400(4):511–516. doi: 10.1016/j.bbrc.2010.08.081

Table 3. Substrate dependent GS activity (kcat)a.

Enzyme GAB + Gly + ATP GAB + ATP % Gly Independent
Wild-type 18.83 ± 0.77 0 0
G369V 0.133 ± 0.001 0.114 ± 0.017 85
G370V 0.052 ± 0.026 0.057 ± 0.024 100
G371V 2.36 ± 0.35 0.116 ± 0.065 5
a

Results are averages of replicate assays of two or three different enzyme preparations. For these studies the background rate subtracted to obtain Kcat values is that of ATP-Mg alone (Enzyme+ATP-Mg). There is no activity with glycine plus ATP.