. Author manuscript; available in PMC: 2011 Oct 1.

Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 26;400(4):511–516. doi: 10.1016/j.bbrc.2010.08.081

Table 4. K_m values for glutathione synthetase mutant^a.

Enzyme	ATP (mM)	Gly (mM)	GAB (mM)^b
Wild-type	0.012 ± 0.009	0.529 ± 0.009	1.26 ± 0.32
Gly369Val	0.44 ± 0.04	N.D.	0.89 ± 0.36
Gly371Val	0.12 ± 0.05	2.52 ± 0.30	0.100 ± 0.043

The results are the averages of replicate assays of two or three different preparations. N.D.: not determined.

GAB is estimated.

Table 4. Km values for glutathione synthetase mutanta.