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. Author manuscript; available in PMC: 2012 Jan 2.

Published in final edited form as: DNA Repair (Amst). 2010 Oct 20;10(1):24–33. doi: 10.1016/j.dnarep.2010.09.005

(A) A pre-incubated solution of Pol λ (120 nM) and 5′-[³²P]-labeled 21/41-mer (30 nM) was rapidly mixed with increasing concentrations of dGTP•Mg²⁺ (0.2 µM, ●; 0.5 µM, ○; 1 µM, ■; 2 µM, □; 5 µM, ▲; 10 µM, △; 25 µM, ◆) for various time intervals. The solid lines are the best fits to a single-exponential equation which determined the observed rate constants, k_obs. (B) The k_obs values were plotted as a function of dGTP concentration. The data (●) were then fit to a hyperbolic equation, yielding a k_p of 0.0353 ± 0.0008 s⁻¹ and a K_d of 0.82 ± 0.08 µM.

(A) A pre-incubated solution of Pol λ (120 nM) and 5′-[³²P]-labeled 21/41-mer (30 nM) was rapidly mixed with increasing concentrations of dGTP•Mg²⁺ (0.2 µM, ●; 0.5 µM, ○; 1 µM, ■; 2 µM, □; 5 µM, ▲; 10 µM, △; 25 µM, ◆) for various time intervals. The solid lines are the best fits to a single-exponential equation which determined the observed rate constants, k_obs. (B) The k_obs values were plotted as a function of dGTP concentration. The data (●) were then fit to a hyperbolic equation, yielding a k_p of 0.0353 ± 0.0008 s⁻¹ and a K_d of 0.82 ± 0.08 µM.