Fig. 1.

LinEs are formed normally in rec24Δ mutants. Diploid pat1-114 cells with the indicated deletions (strains CMC7, CMC40, CMC36 and CMC15) were induced for meiosis, and after 3 hours (prophase), cells were collected for nuclear spread preparation. Spreads were stained with DAPI (DNA; blue) and anti-Rec10 antibodies (red), and photographed under a fluorescence microscope. The fraction of nuclei with Rec10 structures (LinEs) is indicated (n=100 nuclei). Similar results were obtained with chromosome spreads prepared at 3.5 hours after induction and in an independent experiment (unpublished data).