Fig. 2.

Rec24–GFP localizes to LinEs. Diploid pat1-114 rec24-GFP cells (strains CMC197) were induced for meiosis and collected during prophase for nuclear spread preparation. Spreads were stained with DAPI (DNA; blue), anti-GFP antibodies (Rec24–GFP; green) and anti-Rec10 antibodies (red) and photographed under a fluorescence microscope. Rec24–GFP signal was maximal at the time shown (3 hours). Similar results were obtained in two independent experiments. Rec24–GFP signal quantification at 2.5, 3 and 3.5 hours after meiotic induction for one time course is shown in Table 2.