Fig. 6.

Rec7–GFP does not localize to LinEs in rec24Δ mutants. Diploid pat1-114 rec7-GFP cells with the indicated deletions (strains CMC271, CMC272 and CMC273) were induced for meiosis and collected in prophase for nuclear spread preparation. Spreads were stained with DAPI (DNA; blue), anti-GFP antibodies (Rec7–GFP; green) and anti-Rec10 antibodies (red) and photographed under a fluorescence microscope. Stains of chromosome spreads at 3 hours after meiotic induction are shown. No substantial loading was detected in 257 rec24Δ nuclei. Similar results were obtained with 2.5 hour spreads and in an independent experiment (unpublished data).