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. 2011 Mar 28;6(3):e18003. doi: 10.1371/journal.pone.0018003

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Balb/c mice were immunized with Ro60 peptide or PBS (mock) in the tailbase or the abdominal area. Plasma from all animals were analyzed at the indicated time points for anti-MAP-Ro60 antibodies (A). The same mice were analyzed for pilocarpine stimulated salivary flow (B). Data shown are mean values +/- SEM for 10 mice/group. Significant differences are indicated (*** p<0.0001; * p<0.05) and were determined by unpaired Student's t-test. D: number of days post primary injection.