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. 2011 Mar 28;6(3):e18003. doi: 10.1371/journal.pone.0018003

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The SG lymph nodes cells isolated from mice immunized with PBS (mock) or MAP-Ro60 +/- PTX were analyzed by flow cytometry assay for IFN-γ+ and specifically CD4+ IFN-γ+ (Th1) lymphocyte induction 10 days post primary immunization. The SG lymph node cells from one group (N = 10) are pooled and divided into 2 samples. Data shown is from one representative experiment.