Table 4. Bioinformatic analysis of isolated NS1 ED strand-strand dimer characteristics.

Strain	Dimer	Symmetry relationship	Interface accessible surface area (Å2)*	Interface accessible surface area (%)*	Planarity (Å)*	Local density**	Gap Volume (Å3)*	Gap Volume Index (Å)*
PR8	2GX9_ABSS	asymmetric	603	7.7	2.51	32.3	5812	4.82
	3O9U_AF	asymmetric	345	5.2	2.66	22.9	4357	6.31
	3O9U_BD	asymmetric	442	6.6	2.47	25.5	4165	4.71
	3O9U_CH	asymmetric	482	7.3	2.21	30.4	5461	5.67
	3O9U_EG	asymmetric	514	7.7	2.48	31.6	5883	5.73
Mean			477	6.9	2.47	28.5	5135	5.45
Standard deviation			95	1.1	0.16	4.1	817	0.67

ED strand-strand dimers identified as PDB ID_chains (e.g. dimer 3O9U_AF is formed by chains A and F of structure 3O9U). The strand-strand dimer formed by chains A and B of PR8 structure 2GX9 is identified by a superscript SS to distinguish it from the helix-helix dimer also made by those chains (see Table 3). All values calculated using either ProtorP (*) or ProFace (**).