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. 2011 Mar 28;6(3):e18010. doi: 10.1371/journal.pone.0018010

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Durston et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Embryos were injected at the one-cell stage with 16 ng of the MOXMeis3, and analysed by whole mount in situ hybridisation at stage 10.5/11, shown on the left side of each panel, and at stage 12, shown at the right side of each panel. Injected embryos are shown at the bottom of each panel, untreated embryos are shown on top. Shown are vegetal views with dorsal to the top. Expression of Hoxd1 (A), Hoxb4 (B), and Hoxc6 (C) is downregulated in mesoderm of injected embryos at early gastrula stages. A reduction in neurectodermal expression of the three Hox genes studied, is also observed in injected embryos at stage 12.