Figure 5. Synergistic effect between Hoxd1 and XMeis3 in ectopic expression.

Embryos at the one-cell stage were injected into the animal hemisphere with either 100 pg Hoxd1 mRNA, 100 pg Xmeis3 mRNA, or 50 pg of both mRNA's. A single injection of 100 pg of either factor is not sufficient to induce a phenotypic effect. The combination of half the amount of Hoxd1 and XMeis3, results in posteriorisation, shown by a clear reduction of eye formation, and an anterior shift of the eye.