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. 2011 Mar 28;6(3):e18072. doi: 10.1371/journal.pone.0018072

Figure 6. RT43.2GFPBcl-xL vector exposure decreased PC12 cell death induced by serum withdrawal.

Figure 6

A. Undifferentiated PC12 cells on Matrigel-coated wells were transduced with control RT43.2GFP (left four panels) or RT43.2GFPBcl-xL vectors (right four panels) and then serum was withdrawn for either one day (upper panels) or 5 days (lower panels). After either one or five days propidium iodide was used to stain dead cells, and total cell count was obtained by Hoechst33342 staining. The cells were photographed with a 20× objective using either a rhodamine (PI) or DAPI filter. Scale bars = 20 µm. B. Propidium iodide (PI) stained dead cells and Hoechst33342 stained total cells were used to determine the effects of GFPBcl-xL expression (dark purple bar) compared to GFP expression alone (light purple bar) on cell viability. Percentage of PI positive cells versus Hoechst33342 stained total cells is represented by the mean value of several fields obtained from three experiments. *P<0.01.