Figure 8. Differentiated PC12 cells exposed to RT43.2GFPBcl-xL vectors are rendered less susceptible to death attributable to NGF withdrawal.

PC12 cells were plated on Matrigel-coated wells, and treated with 100 ng/ml NGF for five days. Differentiated cells were transduced with RT43.2GFP (grey) or RT43.2GFPBcl-xL (black) vectors during continued treatment with NGF. Three days after infection, the cells were washed with serum-free and NGF-free medium once and fed with serum-free and NGF-free medium for different intervals. PI and Hoechst33342 were added and the cells photographed using a 20× objective using either a rhodamine or DAPI filter. Percentage of PI positive cells versus Hoechst33342 stained total cells is represented by the mean value of several fields obtained from three experiments. *P<0.001.