Figure 10. Transduction of lentiviral vectors into rat cerebral cortical neurons.

Cortical neurons were cultured from rat embryonic brains at day 18 for seven days in vitro and then exposed to 9×10⁵ lentiviral vector particles, comparable to the highest MOI used for gammaretroviral transduction. Five days later, the cells were fixed and immunostained with anti-GFP and TuJ1 antibodies, followed by DAPI staining. The cells were photographed with a laser scanning confocal microscope at 63×. Arrows indicate GFP-positive, TuJ1 positive cells. Scale bars = 3 µm.