Figure 6. Though dissociation-induced PTEN activation is suppressed by Y-27632, it does not contribute to Y-27632 induced increase of the cloning efficiency of prostate stem/progenitor cells.

(A) Western blot analyses of protein expression in prostate colony cells grown with and without 10 µM Y-27632 and in dissociated primary prostate colony cells cultured in suspension for 4 hours with and without 10 µM Y-27632. (B) Bar graph shows the sphere-forming units of WT and PTEN KO prostate cells in the presence and absence of Y-27632. Error bars represent means and STD from 3 wells. * P<0.05, ** P<0.005.