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. 2011 Mar 28;6(3):e18091. doi: 10.1371/journal.pone.0018091

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Sengupta et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Three groups of B6 mice were injected via tail-vein with Ad-WT (open squares), Ad-RGD (open triangles) and PBS (open circles) respectively. Animals were sacrificed at 0, 3, 6, 9, 16, 24, 72 and 96 h after virus injection and spleens were harvested. Splenocytes were surface stained for CD4 and CD8 and then fixed and permeablized and stained with anti-hexon antibody for flow-cytometric analysis of adenovirus infected splenic T cells. Percentage of CD4⁺ T cells expressing adenoviral hexon antigen is represented in upper panel and CD8⁺ T cells are in lower panel. Error bars indicate standard deviation from three animals per group of study. (*p<0.01;**p>0.05; N = 3)