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. Author manuscript; available in PMC: 2012 Mar 25.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Feb 23;406(4):574–579. doi: 10.1016/j.bbrc.2011.02.093

Figure 3. GEFH1 overexpression inhibits podosome assembly and ArfGAP activity.

Figure 3

(A) NIH 3T3 cells were double transfected with plasmids directing expression of Src and the indicated recombinant GEFH1. The number of Src-transfected cells which had podosomes is expressed as the percentage of the total number of cells expressing Src. The data are the summary of three experiments. *, indicates p<0.05 by ANOVA analysis with a Dunnett post test comparing each condition to the GFP control. (B) Expression level of ASAP1 and GEFH1 or ΔCC GEFH1 in cell lysates used for GAP assays were examined. Proteins in the cell lysates were detected by immunoblotting. * in GEFH1 blot indicates faint band of ASAP1 after stripping of ASAP1 antibody from the membrane of upper panel. (C) GAP activity in cell lysates. GAP activity was determined as previously described. The data presented are the summary of 4 experiments. The data were analyzed by ANOVA and a Bonferoni post test comparing activity in the three samples containing ASAP1. *, p<0.05.