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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Gene Expr Patterns. 2010 Dec 21;11(3-4):216–220. doi: 10.1016/j.gep.2010.12.005

Fig. 1.

Fig. 1

In situ hybridization for Sizn1 at E9.5, E12.5 and E14.5 whole mouse embryos. In E9.5 stage, Sizn1 is expressed in dorsal midline of the forebrain (A, B), the ventral rhombencephalon (A, C) and in primodial germ cells (A, D). In E12.5 stage, the expression of Sizn1 is now restricted to the ventral neural tube from the telencephalon through the rhombencephalon (E, lateral view and F, medial view). Expression is found in the lateral olfactory tract (LOT), pallium-subpallium boundary (PSB), amygdala, septum and zona limitans intrathalamica (ZLI). (G) The ZLI expression was confirmed by comparison with Shh-in situ hybridization, which is known to be expressed in the ZLI (Puelles and Rubenstein, 2003). While the pattern of E14.5 is generally similar to that at E12.5, there has been considerable expansion of the expression domains. In a mid coronal section of the whole brain (E14.5) shows strong expression in the amygdala, hypothalamus, ventral thalamus and striatum (H). A lateral (I) and medial (J) mid-sagittal view also shows extensive expression nearly circumferentially in the mesencephalon and rhombencephalon. Am=amygdala, d=diencepahlon, Hy=hypothalamus, m=mesencephalon, Pg=primodial germ cell, r=rhombencephalon, Sp=Septum, St=striatum and VT=ventral thalamus. Scale bar is adjusted to 500 µm in each image.