Fig. 3.

Sizn1 is expressed by Cajal-Retzius cell in the cerebral cortical molecular layer at E14.5 (A). Immunofluorescence for Reln, a marker of early Cajal-Retzius cells, decorates a row of cells in the molecular layer. Labeling with an anti-Sizn1 antibody shows a similar layer of cells and merging of these labels shows complete overlap. DAPI labeling highlights the tissue structure (scale bar = 20 µm). (B and C) Sizn1 is also co-labeled with Reln in the LOT and amygdala. Arrows and arrowhead highlight examples of co-labeled and non co-labeled cell, respectively.