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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: J Chromatogr A. 2010 Nov 16;1218(15):2091–2097. doi: 10.1016/j.chroma.2010.10.107

Figure 2.

Figure 2

AEX at pH 7.5 of XOMA 3AB Antibodies. The two more neutral antibodies (Ab-A, pI=7.4 and Ab-B, pI=6.7) bound to the anion-exchange resin while the basic Mab (Ab-C) flowed through. The force-oxidized Ab-A exhibited two more basic peaks. In contrast, force-oxidized Ab-B shows only a single major peak. Both basic peak fractions of Ab-A were subjected to further characterization by peptide mapping (Figure 4). Force-oxidized Ab-B was also characterized by peptide mapping to determine the sites of modification since no apparent resolution of oxidized species was observed.