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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: J Chromatogr A. 2010 Nov 16;1218(15):2091–2097. doi: 10.1016/j.chroma.2010.10.107

Figure 5.

Figure 5

Tryptic Map of Isolated AEX Fractions from Force-Oxidized Ab-A. The two basic peak fractions (F1 and F2) show a new peak eluting at 86.8 min corresponding to the oxidized Met-containing CDR2 peptide. A concomitant decrease is observed for the peak eluting at 94.1 min. representing the native peptide. The relative peaks heights for the oxidized and native peptides in F1 and F2 are consistent with oxidation of the CDR2 methionine on both (F1) or one (F2) heavy chain subunit(s) in the dimeric antibody.