Table 2.

Human marker genes used in quantitative RT-PCR (F forward, R reverse)

Gene	Primer nucleotide sequence (5′–3′)	Product size (bp)
Aggrecan	F—AGGCTATGAGCAGTGTGACG	125
	R—GCACGCCATAGGTCCTGA
CD45	F—CAGTTTCCCCCATTGACAACC	120
	R—CAGAGGCATTAAGGTAGGCATC
GAPDH	F—TGGACTCCACGACGTACTCA	102
	R—GGAAGCTTGTCATCAATGGAA
IL-1α	F—AACCAGTGCTGCTGAAGGA	97
	R—TTCTTAGTGCCGTGAGTTTCC
IL-1β	F—CTGTCCTGCGTGTTGAAAGA	70
	R—TTGGGTAATTTTTGGGATCTACA
IL-10	F—GATGCCTTCAGCAGAGTGAA	105
	R—GCAACCCAGGTAACCCTTAAA
Leptin	F—TTGTCACCAGGATCAATGACA	71
	R—GTCCAAACCGGTGACTTTCT
Osteopontin	F—GAGGGCTTGGTTGTCAGC	129
	R—CAATTCTCATGGTAGTGAGTTTTCC
TNF-α	F—CAGCCTCTTCTCCTTCCTGAT	123
	R—GCCAGAGGGCTGATTAGAGA
TGF-β1	F—TGGAGACAGGGGCTTTTATTT	121
	R—CTCCAGCCTCCTTAGATCACA