. 2001 Feb 27;98(6):3346–3351. doi: 10.1073/pnas.061020098

Table 2.

NMBA-induced phenotypes in Fhit^+/− mice with and without FHIT gene therapy

Fhit^+/− mice (no. of animals)	Phenotype			Histology of forestomach sections
Fhit^+/− mice (no. of animals)	Esophagus	Forestomach	SCJ	Histology of forestomach sections
Control (12)	1 tumor in 12	≫6 tumors/mouse; some large	Multiple tumors in 8; thickened in 4	FHL in all sections of all mice; papillomas in 11 mice, dys in 3, ca in 1
Ad-FHIT (8)	No tumors	1.7 tumors/mouse	No tumors; thickened in 2	FHL in average of 75% of sections; near normal epithelium in average of 25% of sections
AAV-FHIT (8)	No tumors	<1.4 tumors/mouse; 1 large	No tumors; thickened in 1	FHL in average of 44% of sections; near normal in 56%
Ad-FHIT and AAV-FHIT (6)	No tumors	3.7 tumors/mouse; most small	Tumors in 1; thickened in 1	FHL in 60% of sections; near normal in 40%, ca in section of 1 mouse

At autopsy, whole esophagi and stomachs were removed and opened longitudinally. Esophageal and forestomach tumors with diameters > 0.5 mm were mapped and counted. ca, squamous cell carcinoma; dys, dysplasia. Tumor sizes were graded as follows: large tumors (diameter > 2 mm), medium tumors (diameter 1–2 mm), and small (diameter < 1 mm). Sections, microtome sections cut across SCJ and forestomach. For each forestomach 4–7 sections were examined, in most cases five sections. Near normal epithelium denotes an epithelium that is 3–5 cells thick with mild folding at places and a thin keratin. All control sections show abundant keratin production.