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Agarose gel electrophoresis of Tween 85 niosome/ODN . lane, free OND; lane 2-5, Niosome/ODN with +/− ratio of 0.5:1, 1:1, 2:1 and 4:1 respectively With +/− ratio of 2:1 (Lane 4), slightly strap of OND was visible, while at lane 5 a complete retardation of ODN was performed with +/− charge ratio of 4:1 or above.
