Fig. 3.

FISH Finder's nuclei division function. (a) Results of automated analysis show the segmented boundaries of two nuclei adjoined. (b) The nuclei division function allows users to select two neutral points between adjoined nuclei to be divided. Division points are determined by first selecting the edit button at the bottom of the Editor screen, and then points are chosen by the user on the image itself using the left mouse click button. (Enlarged image shows the two points selected for division as two red crosshairs.) (c) Results of the nuclei division function showing adjoined nuclei divided as two separate nuclear boundaries (left, DAPI; right, FISH signals).