Table 1.
Emission decay parameters for Δ- and Λ-D4 (5μM) measured by time-resolved emission and FLIM in calf thymus DNA (30 μM), phospholipid vesicles (LUV) (150 μM), and Bovine serum albumin (50 μM). Presented data are for air-equilibrated samples.
| Δ enantiomer | Λ enantiomer | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| τ1 (ns) | τ2 (ns) | α2a, f2 | χ2b | τ̄b (ns) | Φc (%) | τ1 (ns) | τ2 (ns) | α2, f2 | χ2 | τ̄ (ns) | Φ (%) | |
| ctDNA | 75 | 670 | 0.55, 0.92 | - | 402 | 3.7 | 20 | 110 | 0.40, 0.79 | - | 56 | 0.4 |
| ctDNA (FLIM) | 83 | 695 | 0.44, 0.87 | 7 | 352 | - | 37 | 292 | 0.11, 0.47 | 4 | 65 | - |
| LUV | 125 | - | - | - | 125 | 1.6 | 136 | - | - | - | 136 | 1.9 |
| LUV (FLIM) | 38 | 218 | 0.25, 0.66 | 9 | 83 | - | 26 | 228 | 0.19, 0.67 | 8 | 64 | - |
| BSA | 30 | 315 | 0.37, 0.86 | - | 135 | 0.3 | 40 | 280 | 0.43, 0.84 | - | 143 | 0.3 |
| BSA (FLIM) | 52 | 534 | 0.15, 0.65 | 29 | 124 | - | 39 | 399 | 0.12, 0.58 | 18 | 82 | - |
a
α2=(f2/τ2)/((f1/τ1)+(f2/τ2))
b
The multi-frequency fit is reproduced by the Levenberg-Marquardt algorithm28, and the goodness-of-fit parameter χ2 is calculated as explained in reference 22 and in Supporting Information.
c
Quantum yields, Φ, are calculated by using the absolute value of 7.72 for Ru(phen)2dppz(CH3)2 2+ in 1,2-propanediol as reference.29
d
Apparent lifetimes are calculated as